Journal: Scientific Reports

Article Title: STK4 protein expression pattern follows different trends in endometrioid and serous endometrial adenocarcinoma upon tumor progression

doi: 10.1038/s41598-022-26391-9

Figure Lengend Snippet: Representative images of the STK4 protein staining in EC tissue. Expression of STK4 was assessed by immunohistochemistry, using a specific antibody. The signal (shown in green and black) was detected mainly in nuclei and in the cytoplasm. Nuclei were counterstained with DAPI. The top row—highly differentiated endometrial carcinoma, the bottom row—middle-low differentiated endometrioid carcinoma. Notice that the STK4 protein signal decreased with tumor progression. CV crystal violet, DNA deoxyribonucleic acid, STK4 serine/threonine-protein kinase 4.

Article Snippet: Figure 4 The STK4 gene expression pattern, obtained at the Broad Institute portal. ( A ) STK4 mRNA expression in various tumor cell lines, according to RNAseq.

Techniques: Staining, Expressing, Immunohistochemistry

Journal: Scientific Reports

Article Title: STK4 protein expression pattern follows different trends in endometrioid and serous endometrial adenocarcinoma upon tumor progression

doi: 10.1038/s41598-022-26391-9

Figure Lengend Snippet: STK4 protein expression in relation to tumor clinicopathological characteristics. The Kruskal-Walli’s test was applied in all cases with respective exact p-values reported in the figure. ( A ) STK4 is expressed at higher levels in Type 1 tumors, than in Type 2 samples; ( B ) STK4 protein levels are decreasing with the advanced tumor grade; ( C ) This observation (in B ) became more clear when serous tumors were included in statistical calculations; ( D ) No significant differences were observed in SKT4 expression, when it was related to the tumor stage; ( E ) A slight decrease in STK4 expression upon tumor progression was detected, when serous tumors were included in calculations.

Article Snippet: Figure 4 The STK4 gene expression pattern, obtained at the Broad Institute portal. ( A ) STK4 mRNA expression in various tumor cell lines, according to RNAseq.

Techniques: Expressing

Journal: Scientific Reports

Article Title: STK4 protein expression pattern follows different trends in endometrioid and serous endometrial adenocarcinoma upon tumor progression

doi: 10.1038/s41598-022-26391-9

Figure Lengend Snippet: STK4 gene expression pattern, obtained at the Oncomine portal. ( A ) STK4 mRNA expression in endometrioid tumors in relation to the grade; ( B ) as in ( A ), related to the stage; ( C ) STK4 gene copy number related to the different stages of endometrioid ECs; ( D ) as in ( C ), but of serous cancers.

Article Snippet: Figure 4 The STK4 gene expression pattern, obtained at the Broad Institute portal. ( A ) STK4 mRNA expression in various tumor cell lines, according to RNAseq.

Techniques: Gene Expression, Expressing

Journal: Scientific Reports

Article Title: STK4 protein expression pattern follows different trends in endometrioid and serous endometrial adenocarcinoma upon tumor progression

doi: 10.1038/s41598-022-26391-9

Figure Lengend Snippet: The STK4 gene expression pattern, obtained at the Broad Institute portal. ( A ) STK4 mRNA expression in various tumor cell lines, according to RNAseq.; ( B ) STK4 mRNA expression according to microarray studies; ( C ) the STK4 gene copy number ratio, calculated based on mRNA expression in normal tissue and cancer cell lines (16). Note that the highly expressed STK4 in Burkitt lymphoma has only 2 copies (20 × 2 = 2), while in solid tumors there is a gain in copy number values (up to 3 copies in EC cell lines: 20.75 × 2 = 3.3); ( D ) methylation of the STK4 promoter, obtained by reduced representation bisulfite sequencing (RRBS). The STK4 promoter methylation is quite high in EC, prostate cancer, and breast cancer cell lines, i.e., in hormone-driven cancers of the human reproductive system. RMA robust multichip averaging, relative units of the signal intensity (see detailed description at https://portals.broadinstitute.org/ccle ). AML acute myeloid leukemia, T-cell ALL acute T-cell lymphocytic leukemia, NSC lung non-small cell lung tumor.

Article Snippet: Figure 4 The STK4 gene expression pattern, obtained at the Broad Institute portal. ( A ) STK4 mRNA expression in various tumor cell lines, according to RNAseq.

Techniques: Gene Expression, Expressing, Microarray, Methylation, Methylation Sequencing

Journal: Scientific Reports

Article Title: STK4 protein expression pattern follows different trends in endometrioid and serous endometrial adenocarcinoma upon tumor progression

doi: 10.1038/s41598-022-26391-9

Figure Lengend Snippet: STK4 expression and 5-year survival of patients with EC. ( A ) STK4 protein expression shows a trend to decrease in Type 2 EC samples of deceased patients, i.e., in poorly differentiated cancers; bars in solid colors represent living individuals, bars with patterns—deceased patients; ( B ) as in ( A ), however, this phenomenon is better recognized in individuals with the Type 2 EC; ( C ) overall survival of patients with endometrioid (left two bars) and serous (right two bars) EC; high (above median) STK4 expression is associated with poor prognosis for patients with serous tumors (pink—% of living patients, blue—% of deceased patients).

Article Snippet: Figure 4 The STK4 gene expression pattern, obtained at the Broad Institute portal. ( A ) STK4 mRNA expression in various tumor cell lines, according to RNAseq.

Techniques: Expressing

Journal: Scientific Reports

Article Title: STK4 protein expression pattern follows different trends in endometrioid and serous endometrial adenocarcinoma upon tumor progression

doi: 10.1038/s41598-022-26391-9

Figure Lengend Snippet: A survival analysis, based on the intensity of a STK4 protein signal.

Article Snippet: Figure 4 The STK4 gene expression pattern, obtained at the Broad Institute portal. ( A ) STK4 mRNA expression in various tumor cell lines, according to RNAseq.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Overexpression of collagen VI α3 in gastric cancer

doi: 10.3892/ol.2014.1910

Figure Lengend Snippet: Network construction of COL6A3 co-expressed genes. The biological interactions of COL6A3 co-expressed genes were analyzed and visualized using a gene expression pattern scanner. The category of each gene is distinguished by its shape for factors, such as kinases and transporters. The direction of the arrow demonstrates whether a gene is upstream or downstream of another gene. Dashed line, co-cited genes; solid line, genes with an expertly curated connection. Genes with no interactions are not shown.

Article Snippet: The gene interaction network was constructed using a gene expression pattern scanner (GePS: http://www.genomatix.de/ ) as described previously ( ).

Techniques: Gene Expression